Page 122 - Catalog Instruments and Supplies

122 SCP SCIENCE
Solid Phase Extraction

Solid Phase Extraction

SPE (Solid Phase Extraction) is a technique whereby a desired interest are not retained. Again, because the potentially interfering
analyte, a cation or an anion, is concentrated and separated from components have been removed, better sensitivity is obtained in
a complex sample matrix onto a sorbent stationary phase. The sample analysis. This technique is seen most often in the food
interfering matrix, which is not retained, is effectively eliminated. As industry.
a result, the analyte can be analyzed at the best possible sensitivity
range of the analytical technique, e.g. ICP-OES, without the risk of In both cases, the sorbent is placed in cartridges or liquid
matrix interference. chromatography columns that can hold varying quantities of
unprocessed samples.
In recent years, another technique, Matrix Component Retention,
has become very popular. In this case, the sorbent is selected to In general, the SPE method should follow 4 steps:
retain unwanted components in the matrix and the analytes of
Step One Conditioning The sorbent is prepared for the
Step Two Retention sample.
Step Three Rinsing
Filter the desired analyte(s) or the
Step Four Elution unwanted matrix components.

Any possible undesired component
that may have been absorbed on the
sorbent is washed away.

The selective desorption and
collection of the analyte of interest
from the cartridge or column.

Typical Applications The Bed Volume and the Retention Capacity

Sample preparation for the analysis of heavy metals in ground water To use the SPE method effectively, choose the cartridge or
column volume and the appropriate sorbent type and mass for
Removal of matrix from food and feed digests for improved the analyte of interest. Normally, the total retention capacity
transition metal elemental analysis (including interferents) of the cartridge or column is approximately
Removal of matrix from blood and serum digests 5% of the sorbent mass.
for improved heavy metal elemental analysis
Concentration and desalination for the analysis of seawater samples
Enhancing the sensitivity of ICP Analysis for water samples through
SPE pre concentration
Weight reduction of water samples for transportation
(carry only collected samples in cartridges)

www.scpscience.com | e-mail: [email protected] | phone: +1 (514) 457-0701